00:01
That consists of 104 amino acid residue.
00:04
In a planning, a solid phase synthesis, we expect to have a 99 .7 % yield for each coupling step.
00:14
So for each coupling step, that means the two amino acid come together.
00:19
So there are 104 amino acid residue.
00:24
There will be 103 coupling steps.
00:26
Now, a 99 .3 % yield for each deblocking.
00:33
So each deblocking step also yields 99 .3%.
00:37
Now there are 104 amino acid residues.
00:40
There will be a total of 104 deblocking steps.
00:47
And then the cleavage of the completed polypeptide from the resin and the side chain deblocking step yield 80 % of recovery.
01:00
So it asks you, what is the yield of the protein if we synthesize entire protein in a single run? so the very first step, we call this coupling step.
01:18
The coupling step has 99 .7%...