What are the differences in how an indirect and a direct ELISA work? How is the testing process different? Why do we need 2 tests?
Added by Ronald H.
Step 1
** Show more…
Show all steps
Your feedback will help us improve your experience
Adi S and 91 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
What is the difference when comparing the rapid-antigen ELISA test and the antibody ELISA test? How is it set up differently, and what are the different methods?
Adi S.
You conduct a side by side comparison of a direct and indirect ELISA for the presence of the same virus. The results of the direct ELISA are negative, indicating that the viral antigen is not present in the patient serum. The results of the indirect ELISA are positive, indicating that antibodies against the virus are present. Why do you see two different results, even those they are designed to detect the same virus? (Assume that the controls worked in the assay)
In an indirect ELISA assay, which is the capture molecule, antigen or antibody? Why?
Madhur L.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD