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Hello everyone.
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So in this particular question, we are asked about the two factors that affect the resolution of light microscope.
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And the second part of the question asks us that oil, it increases resolution.
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So we need to tell that how and why this happens.
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So let's first deal with the first part of the question that is two factors that are affecting resolution.
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But before that, let's understand that what resolution means.
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So we can say that resolution, it is the ability, right? suppose there is a specimen and it is having specific structures which we need to see under the microscope.
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So if two structures are located at a distance and the microscope is able to segregate those two objects with the distance that they are, so that is known as resolution.
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That means each object is seen clearly with the distance.
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From the other objects.
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So hence we can write down that the ability to separate or segregate, right? or we can say the ability to segregate two objects or we can say two points, it might be a structure or object inside the specimen distance apart.
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So this is known as resolution.
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Now we need to understand that what are the two factors? factors that can affect the resolution of a light microscope.
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So the two factors here are the first one is the numerical aperture, right? so the first is the numerical aperture.
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So this can affect, we can say, the resolution of a light microscope.
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Now how it does that? so we can say that higher the numerical aperture, right? i'll write it as n .a.
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Short form for numerical aperture.
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So higher the numerical aperture, better would be the resolution.
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Right? better would be the resolution...