What different biochemical test could you use to determine whether your microbe ferments lactose, sucrose, or both?
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When investigating an unknown microorganism for lactose fermentation, why would you use two methods (MacConkey's Agar and lactose fermentation broth tubes)? How are they different in terms of the range of microorganisms you can use them for and why?
Sri K.
What test could be used to differentiate between sucrose and lactose? Explain.
Hitendra S.
If you have a yellow slant and a yellow butt on TSIA, can you tell if the organism can ferment sucrose only or lactose only? If not, which other test can you use to be more specific about sugar fermentation? Yes, this test identifies whether an organism can ferment only sucrose or only lactose. You would not need to perform any further tests. No, this test will not determine if the organism is only a sucrose or only a lactose fermenter. You would need to perform the phenol red broth test with the individual sugars to determine which sugar the organism can ferment. No, this test will not determine if the organism is only a sucrose or only a lactose fermenter. You would need to perform the LIA test to determine which sugar the organism can ferment. Yes, this test helps to identify whether an organism can only ferment sucrose or only ferment lactose. You could perform the phenol red broth test to confirm sugar fermentation. None of the above
Rupsa S.
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