What does MSA (Mannitol Salt Agar) test for? selective for gram - or +? Differential for what? And how do you interpret the results?
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Answer the following questions about Mannitol Salts Agar (MSA) a. MSA is both selective (due to high salt concentration) and differential (allows us to see if the bacteria is able to ferment mannitol). Some bacteria are not able to grow in the high salt environment of MSA (for example, Micrococcus luteus). Can we be sure if these bacteria are able to ferment mannitol or not? Explain your answer. b. What if we left out the mannitol: would it still be selective? Differential? Explain your answer. c. If we left out the high NaCl of the MSA media, would it still be selective? Differential? Explain your answer. 5. Is the blood agar media selective and/or differential? Explain your answer.
Adi S.
Selective and Differential Media: MSA Is MSA selective, differential, or both? What are the selective ingredients? What is the differential ingredient? What is the pH indicator? What does this media select for? What does this media differentiate? Is quadrant 1 positive or negative for mannitol fermentation? Give an example of an organism (genus + species) that would give the reaction seen on this quadrant. Is quadrant 2 positive or negative for mannitol fermentation? Give an example of an organism (genus + species) that would give the reaction seen on this quadrant. Bacteria was inoculated onto quadrant 3. Why didn't it grow? Give an example of an organism (genus + species) that would give the reaction seen on this quadrant.
Sri K.
Lab 4-2 Blood Agar (BA) 1. What chemical or property makes the media selective? How is it selective? 2. What chemical or property makes the media differential? How is it differential? (Note color changes to the media, for example, MSA is pink at the start and can turn yellow) 3. Does the media select for or against Gram-positive and Gram-negative bacteria? (Remember that "selecting for" means it promotes the growth of" and "selecting against" means it suppresses the growth.) Lab 4-3 Mannitol Salt Agar (MSA) 1. What chemical or property makes the media selective? How is it selective? 2. What chemical or property makes the media differential? How is it differential? (Note color changes to the media, for example, MSA is pink at the start and can turn yellow) 3. Does the media select for or against Gram-positive and Gram-negative bacteria? (Remember that "selecting for" means it promotes the growth of" and "selecting against" means it suppresses the growth.)
Josee P.
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