00:01
We've been given a peptide and we need the amino acid sequence.
00:04
We're going from the n to the c terminus.
00:07
So first we have to find the n terminus is at the top or the bottom.
00:12
Well, the top has an exposed nh3 plus.
00:17
This is the n terminus.
00:19
So that's where we're starting off.
00:20
We can see it's connected to its carbon hydron.
00:25
This is the carbonile.
00:28
And this here is our first.
00:32
Side chain.
00:35
So this is just the top.
00:36
So we've got the amino group here, central carbon, amino, central carbonile, and this here is the side chain.
00:48
So that's what we're starting.
00:50
Okay.
00:53
So what is that side chain? give me some space to write out the answers.
01:03
Okay, so we have a c -h -2 and then an o -h.
01:09
So that is serene.
01:13
So if i draw it as a skeleton instead, we have the central carbon here, and it comes out like this.
01:22
This is serine.
01:25
Okay, next one.
01:28
So if we go past the carbon oil, we see another amino group, another central carbon, and we have c -h2 into so this is the next one.
01:40
Sulfur, well only two acts we have sulphur in, and that's methionine and cysteine.
01:47
Which one is this? this one is cysteine.
01:51
Methyane has it in the middle of its chain, so methionine, instead of the hydrant here, would continue out into a methyl group.
02:00
But this one is cysteine, because it has the solfer at the end of its chain.
02:05
So just notice it wants the letters, so serine is s, cysteine is c.
02:14
Okay? so there are 20 amino acids, and honestly if you have to memorize the structures, flashcards are a really good idea.
02:27
Going on to the next one, we have a carbon here, and it splits off into two...