00:01
What is the difference between deoxynucleotide and dideoxynucleotide? so i'm going to draw the structure of the two different nucleotides and you can tell the difference.
00:14
So let's start with the regular dntp.
00:24
So the dntp, deoxyribonucleotide, start out with a 5 -carbon sugar, and one side connects to 3 -phosphate group, and the other side connects to a nitrogen base, so one of the atcg.
00:56
So this is number 1 of carbon, 2, 3, 4, and 5th carbon.
01:04
So we can see that the phosphate is connected to the 5th carbon.
01:08
Now at the nitrogen base is connected to the 1st carbon.
01:11
At the 2nd carbon you have a h, hydrogen ion, and at the 3rd carbon you have a oh, hydroxide group.
01:21
So this is a regular dntp.
01:25
Now let's take a look at the d -dntp.
01:30
So they have similar structure for the most part, so you have a 5 -carbon ribose, and at the 5th carbon, again, there are 3 -phosphate group, and at the 1st carbon you still have one of the 4 nitrogen base, and at the 2nd position you still have a h.
02:00
However, at the 3rd carbon, now instead of having a h, it connects with an oh, it has a h...