4. What is the function of the mordant in this procedure? 5. Why is it important to use young cultures for accurate Gram stain results? 6. What is the critical step in the Gram stain procedure? Why? 7. The difference between Gram (+) and Gram (-) bacteria lies in what bacterial cell structure? 8. In the lab, distilled water was used instead of ethyl alcohol as the decolorizer. Would this change the results of Gram (+) bacteria? Gram (-) bacteria? Explain.
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The function of the mordant in the Gram stain procedure is to help the primary stain (crystal violet) bind more effectively to the bacterial cell wall. This is typically achieved using iodine, which forms a complex with the crystal violet, making it more difficult Show more…
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