1. What is the major difference between western blot and Immunohistochemistry? 2. What is the difference between a monoclonal and polyclonal antibody? 3. When is antigen retrieval necessary? 4. What is the developing reagent used for immunohistochemistry in this weeks practice? How this reagent different from the substrates used in ELISpots and ELISA assays?
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Monoclonal antibodies are made from a single type of antibody, while polyclonal antibodies are made from multiple types Show more…
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The differences between the three immunoblotting techniques are: A. All three assays use a membrane. B. Elisa and Dot-Blot assays use a membrane, while Western blotting does not use a membrane. C. Elisa and Western blotting use electrophoresis, whereas the Dot-blot does not need electrophoresis. D. Dot-Blot and Western blotting can detect the molecular size of unknown proteins, but Elisa assay cannot. E. Dot-Blot and Western blot use a membrane, whereas the Elisa assay does not have this step.
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Choose the answer that best describes a difference between Monoclonal and Polyclonal antibodies. Monoclonal antibodies are delivered through a blood transfusion, polyclonal antibodies are delivered through an intramuscular injection Monoclonal antibodies are made from T cells, polyclonal antibodies are made from B cells Monoclonal antibodies are made from identical B cell clones, Polyclonal antibodies are derived from blood plasma of an infected patient A monoclonal antibody refers to 1 very strong antibody given to a patient, polyclonal antibodies are where a patient receives several weaker antibodies
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