What were the main materials you used and what are their...

What were the main materials you used, and what are their major properties? Specifically mention the 2 plasmids that were used, the main restriction enzymes and other enzymes used and their properties.

In your research you are given two tubes of DNA: Tube 1 contains a purified PCR amplified human beta-globin locus that is about 2kb in size. This PCR product has intact Not I restriction sites flanking the gene. Tube 2 contains a 5.2 kb circular plasmid vector, pcDNA3.1 (-), containing a multi cloning site with recognition sites for Nhe I, Pme I, Not I, Eco RI, Hind III, and Pme I. This vector has the Neomycin and Ampicillin resistance genes. You want to clone the beta-globin gene into the pcDNA3.1 (-) vector. Describe the following: a) How you will ligate the beta-globin gene insert into the pcDNA3.1 (-) vector to create a recombinant molecule? (6 marks) b) How you will introduce this ligation mix containing the recombinant molecule into a host E. coli strain? (4 marks) c) How you will select for the transformants containing the recombinant DNA in the E. coli? (2 marks)

Adi S.