What would be the functional effect on transcription of the lac operon if there was a stop mutation (change to a stop codon) at the beginning of the Lacl gene (gene that codes for the repressor protein), resulting in no protein being produced from that gene? Select one: a. operon would function normally – only on when lactose is present b. operon always off – cannot be induced to turn on c. constitutive transcription (operon is always on)
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Step 1: A stop mutation at the beginning of the LacI gene would result in no repressor protein being produced. Show more…
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A mutation occurs in the lac repressor protein such that it can no longer bind to lactose. What effect will this mutation have on the expression of proteins of the lac operon in the presence of lactose? a. It will have no effect since lactose shuts off the operon. b. It will decrease the expression of the lac operon proteins. c. It will increase the expression of lac operon proteins. d. It will have no effect since the operon is modulated by another protein.
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Mutations in any of the genes involved in lac operon will lead to loss of functionality. What happens if the lacI gene is mutated and always OFF? A) The lacY gene will not be transcribed and translated. B) There will be continuous transcription of the lacI gene. C) The lacI protein (the repressor) will be bound to the lac operator, even in the presence of lactose (the inducer). D) There will be continuous transcription of the lac operon genes, even in the absence of lactose, (the inducer).
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A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription
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