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When double-stranded DNA is heated to 100°C, the two strands separate because the hydrogen bonds between the strands break. When the solution is cooled, the two strands can find each other to re-form the double helix, a process called renaturation or reannealing. For example, consider the following DNA double helix: 5' - GCGCGCGCGCGCGC - 3' 3' - CGCGCGCGCGCGCG - 5' Now, imagine that this DNA is heated to 100°C and then cooled, but the two separated strands never find each other. The local secondary structure of each single strand might assume the shape of a hairpin loop.

          When double-stranded DNA is heated to 100°C, the two strands separate because the hydrogen bonds between the strands break. When the solution is cooled, the two strands can find each other to re-form the double helix, a process called renaturation or reannealing. For example, consider the following DNA double helix:
5' - GCGCGCGCGCGCGC - 3'
3' - CGCGCGCGCGCGCG - 5'
Now, imagine that this DNA is heated to 100°C and then cooled, but the two separated strands never find each other. The local secondary structure of each single strand might assume the shape of a hairpin loop.

Added by Adri-N S.

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