When performing the protein assay using bovine serum albumin (BSA) as the protein standard, what amino acids does the Bradford Protein Assay primarily measure? Basic amino acids Acidic amino acids One alanine Sulfur amino acids
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13) In this chapter, you read that the Bradford assay has a higher sensitivity to some proteins than other proteins and hence gives higher absorbance readings at 595 nm. For example, the absorbance readings for bovine serum albumin are more than twice those for bovine gamma globulin (Figure 3.6). 14) In the Bradford assay of a protein, what is the most ideal protein to use as a standard?
Rebecca C.
4. Coomassie Blue binds to basic amino acids and to aromatic residues. Since the number of these types of amino acids within a protein determines how much dye will bind to a protein, a Bradford assay is usually operated under the assumption that the unknown protein will have a similar amino acid composition as the standard BSA. However, this is not always the case. How would your results be changed if the unknown protein had a higher percentage of basic and aromatic amino acids? Would your results be artificially lower or higher?
Adi S.
David C.
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