Question

When you analyze the DNA sequence of the specimen, you determine the specimen is Escherichia coli 0157:H7. However, further analysis reveals the gene for the Shiga toxin from a different type of bacteria known as Shigella dysenteriae. 1.3a) When examined under a high-power microscope a sex pilus is not observed. Which of the 3 mechanisms of gene transfer is NOT responsible? Sex pili or conjugative pili allows the gene through the process of bacterial conjugation. Absence of sex pili indicates that conjugation was absent. 1.3b) When examined under a high-power microscope, a bacteriophage was seen on the surface of the E. coli cell. Which of the 3 mechanisms of gene transfer is MOST likely responsible for the transfer of the Shiga toxin gene from Shigella dysenteriae to Escherichia coli. 1.3c) Explain what type of gene transfer is this and why? 1.3d) Create a labeled diagram and describe the gene transfer process by which the Shiga toxin gene was picked up from Shigella dysenteriae and transferred to Escherichia coli. Be as specific as possible in your description of this process. Meaning be sure to label your donor and recipient cells as the bacterial species given depending on how the genes are being transferred.

          When you analyze the DNA sequence of the specimen, you determine the specimen is
Escherichia coli 0157:H7. However, further analysis reveals the gene for the Shiga toxin from
a different type of bacteria known as Shigella dysenteriae.

1.3a) When examined under a high-power microscope a sex pilus is not observed. Which of the
3 mechanisms of gene transfer is NOT responsible?

Sex pili or conjugative pili allows the gene through the process of bacterial conjugation.
Absence of sex pili indicates that conjugation was absent.

1.3b) When examined under a high-power microscope, a bacteriophage was seen on the surface
of the E. coli cell. Which of the 3 mechanisms of gene transfer is MOST likely responsible for
the transfer of the Shiga toxin gene from Shigella dysenteriae to Escherichia coli.

1.3c) Explain what type of gene transfer is this and why?

1.3d) Create a labeled diagram and describe the gene transfer process by which the Shiga
toxin gene was picked up from Shigella dysenteriae and transferred to Escherichia coli. Be as
specific as possible in your description of this process. Meaning be sure to label your donor and
recipient cells as the bacterial species given depending on how the genes are being transferred.

When you analyze the DNA sequence of the specimen, you determine the specimen is
Escherichia coli 0157:H7. However, further analysis reveals the gene for the Shiga toxin from
a different type of bacteria known as Shigella dysenteriae.

1.3a) When examined under a high-power microscope a sex pilus is not observed. Which of the
3 mechanisms of gene transfer is NOT responsible?

Sex pili or conjugative pili allows the gene through the process of bacterial conjugation.
Absence of sex pili indicates that conjugation was absent.

1.3b) When examined under a high-power microscope, a bacteriophage was seen on the surface
of the E. coli cell. Which of the 3 mechanisms of gene transfer is MOST likely responsible for
the transfer of the Shiga toxin gene from Shigella dysenteriae to Escherichia coli.

1.3c) Explain what type of gene transfer is this and why?

1.3d) Create a labeled diagram and describe the gene transfer process by which the Shiga
toxin gene was picked up from Shigella dysenteriae and transferred to Escherichia coli. Be as
specific as possible in your description of this process. Meaning be sure to label your donor and
recipient cells as the bacterial species given depending on how the genes are being transferred.

Added by Miguel H.

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