5. Which areas of your hands were the cleanest after washing, and which areas had the most Glo Germ? (2 pt) Cleanest areas: hand Most Glo Germ: Fingers 6. Indicate the sites where microorganisms are likely to be found. (Select all that apply) (1 pt) a. Laboratory bench surface b. Hands c. Surface of sterile agar plate d. Computer keyboard 7. Why can most microbes NOT be cultured in a laboratory? (1 pt) a. This statement is false - any microbe can be grown in a lab. b. The materials needed to grow most microbes are prohibitively expensive. c. Most microbes isolated from the environment die before reaching the lab. d. The conditions needed to grow most microbes are unknown or unable to be replicated. 8. Which of your sources for microbes resulted in the most growth? Which had the least growth? (1 pt) Most growth: Least growth:
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If you were to make Columbia CNA with 5% sheep blood agar plates with twice as much colistin, how would the results likely be affected? Select one: a. Less Gram-positive growth, more Gram-negative growth b. Less Gram-positive growth, no or even less Gram-negative growth c. More Gram-positive growth, more Gram-negative growth d. More Gram-positive growth, no or even less Gram-negative growth e. Same Gram-positive growth, no or even less Gram-negative growth 2. In Mannitol Salt Agar lab, you were told to invert the plate before incubation. This is a common practice to avoid: Select one: a. Condensation drops b. Increased smell c. Decreased oxygen content d. Bacteria from growing in the wrong direction e. Airborne contamination 3. If you see a yellow halo surrounding a colony on an MSA plate, which of the following is likely to be true? Select one: a. Glycolysis occurred b. The organism is Staphylococcus epidermidis c. Colonies are not isolated d. Fermentation occurred e. Colony is Gram-negative 4. Which one of the following is found in MSA plates, but not in NA plates? Select one: a. Phenol red b. Agar c. Tryptose d. Peptone e. Beef extract 5. Why are the three organisms of Phenylethyl Alcohol Agar lab inoculated on the same plate? Select one: a. To avoid contamination b. For easy viewing purposes c. To save resources d. To avoid fluctuations in medium content and environmental effects 6. For which reason would you most likely use a PEA plate for culturing after taking a sample from a patient? Select one: a. To isolate Streptococcus and Staphylococcus from other fecal bacteria b. To detect Escherichia contamination on the skin c. To find E. coli strains associated with food poisoning d. To identify gram-negative anaerobes in the patient's flora
Sri K.
Techniques/procedures designed to prevent microbial contamination of sterile equipment, drugs, patients, or pure cultures are called sterile/aseptic techniques.
a. Which of the following individuals discovered the microbial basis of fermentation and later used it to refute the idea of spontaneous generation? (A) Florence Nightingale (B) Antoni van Leeuwenhoek (C) Louis Pasteur (D) Robert Hooke (E) Robert Koch b. Which of the following individuals discovered the antibiotic penicillin? (A) Robert Koch (B) Alexander Fleming (C) Lazzaro Spallanzani (D) Joseph Lister (E) Edward Jenner c. Which of the following is a difference between antiseptics and antibiotics? (A) Antiseptics kill microbial cells but antibiotics do not. (B) Antibiotics kill microbial cells but antiseptics do not. (C) Antibiotics may be taken internally but antiseptics cannot. (D) Antiseptics can be taken internally but antibiotics cannot. (E) none of the above d. According to the endosymbiosis theory, mitochondria evolved from ancient (A) fungi (B) cyanobacteria (C) proteobacteria (D) archaea (E) respiring protists e. Which of the following were critical for Carl Woese to develop the three-domain scheme of classification? (A) germ theory of disease (B) sequences of 16S rRNA (C) advances in light microscopy (D) petri dishes for pure cultures (E) ultracentrifugation techniques f. The genetic expression machinery of archaea is most similar to that of? (A) monera (B) prokaryotes (C) eukaryotes (D) bacteria (E) mitochondria g. A bacteriophage is (A) a method bacteria use for feeding (B) a type of archaea (C) a virus that infects eukaryotic cells (D) a virus that infects bacteria (E) a bacterium that consumes viruses h. Which of the following would allow a disease-causing microbe not to adhere to Koch's postulates? (A) The microbe is found in all patients with a particular disease but also in some patients without the disease. (B) The microbe can infect multiple different species, for example both humans and rabbits. (C) The microbe can be grown in pure culture. (D) The microbe is a eukaryotic pathogen. (E) The microbe can be killed by antiseptics. i. Which of the following does NOT contain DNA or RNA? (A) prokaryote (B) eukaryote (C) virus (D) viroid (E) prion
Jorge V.
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