00:01
So two eukaryotic proteins are being analyzed, and the amino acid sequencing demonstrates that the proteins vary greatly except for one conserved domain.
00:09
So what is the most likely cause for the sharing of this domain? would it be a, a point mutation recently occurred in one of the genes? b, alternative splicing of the same gene results in two protein products.
00:23
C, one of the genes was recently duplicated, or d, exon shuffling occurred causing the shift.
00:30
Of a small conserved domain.
00:32
So if we draw out our gene as it is in the dna, we'd have our, we'd have some of the chromosome that wasn't involved in the gene, then once the gene comes in, we would have an exon, so i'm going to put the exons in green.
00:55
You might have some introns, a few more exons, and introns, then of course you're just going to have the rest of the chromosome.
01:28
So when dna is being converted into rna, you can have different recombinations of the gene.
01:53
So here we'll have x -on -1, 2, 3, 4, 5, 6.
02:05
Say one version of the rna might have one, two, four, five, six, and another might have one, two, might have one, two, three, five, six, etc.
02:30
But you can see that if alternative splicing were to occur, chances are that a lot of the protein is going to be conserved.
02:40
So here in this hypothetical scenario, 1, 2, 5, and 6 are conserved, and only one small region defers.
02:53
So it's probably not alternative splicing.
02:56
So we can cross that out.
03:05
If it were a point mutation, so if there were, say, a point mutation right here, it would be, maybe one amino acid change or something like that, which again it would be a small region that's differing between proteins rather than one small region that is conserved between proteins...