Which of the following does not contribute to carbamoyl phosphate? O Phosphate from ATP O An acetyl group from acetyl-CoA O A carbonyl from carbon dioxide O Free ammonia O All of the above do contribute to carbamoyl phosphate.
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Step 1: Write the overall reaction for carbamoyl phosphate synthesis (carbamoyl phosphate synthetase I): 2 ATP + HCO3- + NH3 → carbamoyl phosphate + 2 ADP + Pi. Show more…
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QUESTION 13 The following reaction is catalyzed by the enzyme Carbamoyl phosphate Synthetase II: Carbonate + 2 ATP + Glutamine + H2O -> Carbamoyl phosphate + Glutamate + 2 ADP + Pi + H+ Carbamoyl phosphate Synthetase generates the HEI carbamoyl phosphate. It is considered an ‘intermediate’ even though it’s shown as the product of the reaction. Carbamoyl phosphate is used to synthesize N-carbamoylaspartate in subsequent reactions to ultimately generate the pyrimidine bases of DNA/RNA. (a) Why is carbamoyl phosphate considered a HEI? (b) There are two other intermediates formed in this reaction, carbonate-phosphate anhydride and carbamate. Which has higher energy? Why?
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