Which of the following is NOT part of the traditional enzyme-linked immunosorbent assay (ELISA)? Choose one: A. Excess antigen is added after IgG addition to encourage cooperativity of the reaction. B. Antigen is attached to the plate’s well bottom and walls, where the reaction will take place. C. Secondary IgG antibody with attached (conjugated) enzyme is added to the reaction. D. Production of a colorful product is produced, that is proportional to the amount of primary antibody.
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