00:01
Okay, so for any bacteria's slide heat fixation is done after its plating.
00:07
So it plays multiple functions.
00:10
So we look at these functions one by one.
00:12
The first function it plays is killing the bacteria.
00:16
So killing bacteria.
00:18
Now it involves passing the slide through the open flame and apply heat to the slide.
00:27
So applied heat is applied and this would rapidly kill the bacteria.
00:33
So rapidly kill bacteria and of this do the function by protein disruptions.
00:41
Proteins of bacteria are disrupted due to that heat.
00:46
So proteins are disrupted and cellular functions stop.
00:49
So this would also stop further growth or replication of bacteria on the slide.
00:55
So growth and replication both are stopped and this would be fixed.
01:03
So the bacteria in the state would be fixed on the slide.
01:07
Second would be attachment to the slide.
01:09
So attachment to slide.
01:13
Now how is that done? that fixation helps bacteria to adhere.
01:18
So it causes adherence to the surface, adhere to the surface of the slide.
01:24
Now it causes bacteria, so bacteria protein, bacteria protein to have other cellular components.
01:35
So other cellular components of that bacteria components to be attached...