1. Which of the following type(s) of media will allow Staphylococcus to grow A. MacConkey Agar B. EMB agar C. TSA D. MSA Agar AB. A and B AD. A and C CD. C and D 2. Which type of bacteria grows well on EMB? A. Lactose fermenters B. Gram Positives C. Mannitol fermenters D. Salt tolerant organisms E. Gram-negatives 3. The high acid production from fermentation of lactose will produce green colonies on this type of plate A. TSA B. EMB agar C. MSA agar D. MacConkey Agar 4. The change of the pink agar in MSA plates to yellow is caused by what? A. The production of a toxin B. The bacteria absorb and neutralize the pink color. C. The pH change caused by fermenting the sugar present in the plate D. None of these. MSA plates do not turn yellow 5. Enterobacter aerogenes plated on MacConkey Agar and EMB agar would be what color A. Pink to Red opaque on both B. Pink opaque and Green opaque C. Green opaque and Pink opaque D. Translucent and Translucent light colored purple 6. Which type of agar plate is differential based on the presence of mannitol as a sugar for fermentation A. MSA agar B. TSA C. MacConkey Agar D. EMB agar
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Staphylococcus can grow on MSA Agar (Mannitol Salt Agar) because it is a salt-tolerant organism and can ferment mannitol. So, the answer for the first question is **D. MSA Agar**. Show moreā¦
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You inoculated a MacConkey agar plate with a gram-positive and a gram-negative bacterial strain. When you observe the plate after incubation, you notice that only the gram-negative bacteria formed colonies. In addition, you notice there is a color change in the media (red to yellow), indicating that this gram-negative bacterium is capable of lactose fermentation. What type of media is this? a) selective b) Differential c) All of the above 2. Fannie Hesse developed the idea of replacing gelatin with agar. What are the benefits of using agar over gelatin? a) Agar is cheap and a good solidifying agent b) Gelatin melts at a lower temperature (40 degrees Celsius), whereas agar does not. c) Agar is a great nutrient source for most bacteria. d) Answer A and B are correct 3. You have been instructed to measure an overnight culture for bacterial density. Which of the following methods would you use if you do not care about distinguishing dead from live bacterial cells? a) Membrane filtration method b) Turbidity c) Most probable number d) Plate count using the spread plate method e) Plate count using the pour plate method.
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1. What is the resulting zone of inhibition if antimicrobial disks were implanted 20 minutes after inoculation? A. larger than the true value B. true value C. smaller than the true value D. cannot be determined 2. Aside from blood agar, which culture medium enables the observation of hemolytic property of Staphylococcus aureus? A. Mannitol agar medium B. Colistin-Nalidixic acid agar C. Vogel-Johnson Medium D. Phenylethyl alcohol agar 3. Which of the following primary culture media gives growth advantage to Group D Streptococci from fecal specimens? A. Blood agar media B. Colistin Nalidixic acid C. Chocolate agar D. Phenylethyl alcohol plate 4. Test that differentiates Moraxella from gonococci is: A. catalase test B. oxidase test C. superoxol test D. penicillin disk test 5. Which of the following are not supported by unsupplemented chocolate agar? A. N. gonorrhoeae and N. meningitidis B. N. meningitidis and N. mucosa C. N. mucosa and N. lactamica D. N. gonorrhoeae and N. lactamica 6. Which is the preferred transport system for cotton swab collected genital specimen suspected with pathologic Neisseria? A. Semisolid media with activated charcoal B. Apotex semisolid C. Stuart's semisolid D. Amie's buffered semisolid
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