7. Which of the following would be killed if placed in an environment that totally lacks oxygen? Circle all
that apply.
a) Obligate aerobe
b) Microaerophile
c) Facultative anaerobe
d) Aerotolerant
e) Obligate anaerobe
8. A tube of thioglycollate broth (inoculated with a bacterial species and incubated) has turbidity only in
the lower half of the broth. At the surface of the broth, the broth has a pink coloration. What is the
oxygen requirement category of this species (circle one)?
a) Aerotolerant
b) Facultative anaerobe
c) Microaerophile
d) Obligate aerobe
e) Obligate anaerobe
9. A bacterial species grows well on an aerobic plate and grows just a little bit on an anaerobic plate.
Based on this, you can categorize this species as (circle one):
a) aerotolerant
b) a facultative anaerobe
c) an obligate aerobe
d) a microaerophile
e) an obligate anaerobe
10. Which of the following is an oxygen requirement category you could determine using thioglycollate
broth, but NOT using the combination of aerobic+anaerobic plates? (hint: try to draw expected results
for these two different ways of assessing oxygen requirement categories!) (circle one)
a) facultative anaerobe
b) obligate anaerobe
c) obligate aerobe
d) microaerophile
e) aerotolerant
11. In the absence of oxygen, what are TWO other ways that bacteria can make ATP (not including
photosynthesis)? What is the difference between these 2 (hint: your answer should include the phrase
"electron transport chain")?