Which region associated with the lac operon is not involved in the binding of RNA polymerase? Multiple Choice CAP binding site laco lacz
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The lac operon is regulated by a repressor protein that binds to the operator region of the operon. When lactose is present, it binds to the repressor protein, causing it to detach from the operator. This allows RNA polymerase to bind to the promoter region and Show more…
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Which region(s) of the lac operon affect the binding of RNA polymerase? (Select all that apply) CAP binding site lacO lacZ lacY lacA
Farhan A.
1. The lac operon in E. coli comprises three structural genes, lac Z, lacY, and lacA. It is regulated by both positive and negative mechanisms, involving binding of both a repressor protein and the catabolite activator protein (CAP). Transcription of the operon yields a polycistronic mRNA encoding the three enzymes. Under the following nutritional conditions, indicate whether or not the repressor and the CAP are bound (yes/no), and if bound, to which region(s) of the operon (write the corresponding letter and symbol – e.g., Z+ if bound to the lac Z gene). Also indicate whether or not a messenger RNA is made by indicating whether the expected amount of product is: none, low, medium, high. a. When lactose and glucose are absent: |_p_| i+ |_p+|_o+| z+ | y+ | a+ | Repressor bound? ________ to: ________ CAP bound? ________ to: ________ Expected amount of product: ________ b. When lactose is present and glucose is absent: |_p_| i+ |_p+|_o+| z+ | y+ | a+ | Repressor bound? ________ to: ________ CAP bound? ________ to: ________ Expected amount of product: ________ c. When both lactose and glucose are present: |_p_| i+ |_p+|_o+| z+ | y+ | a+ | Repressor bound? ________ to: ________ CAP bound? ________ to: ________ Expected amount of product: ________
Question 11 (1 point) The lacP site within the lac operon is upstream of the genes that encode for lactose uptake and metabolism proteins. The lacP site is Question 12 (1 point) Small effector molecules
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