While Gram-staining , you are worried baout adding too much decolorizer and only leave the solution on for a couple of seconds. Although you thought you had a Gram-negative species, the results appear Gram-positive. What happened?
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Your IA gave you two bacterial cultures to practice gram staining on. She said one of the tubes contained gram-negative and one contained gram-positive bacteria. You stained both on the same slide for comparison and saw that both your bacteria appeared pink. What could have gone wrong? Please select all that apply. - Gram-positive cells were too old. - You decolorized your slide longer than required. - Your IA has mixed up the cultures. - Gram-negative bacteria were too old. - For the primary stain, you used safranin instead of crystal violet.
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You have prepared a smear and performed the Gram stain. Your TA told you that you had a Gram-negative organism and Gram-positive but all you see are purple cells. What could have gone wrong with your staining technique?
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Question: A smear of gram-negative bacteria has given gram-positive results. Provide two procedural reasons as to why this incorrect reaction may have occurred.
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