Why should young cultures be used when doing a Gram stain? Group of answer choices The peptidoglycan layer in old cells does not retain iodine well The peptidoglycan layer in old cells does not retain carbol fuschin well The peptidoglycan layer in old cells does not retain crystal violet well
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The stain involves the use of crystal violet, iodine, a decolorizing agent, and a counterstain (usually safranin). Show more…
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Why must young cultures be used when doing a Gram stain? What part of the bacterial cell is most involved with Gram staining, and why? What is the function of the iodine solution in the Gram stain? If it were omitted, how would staining results be affected? What is the purpose of the alcohol solution in the Gram stain? What counterstain is used? Why is it necessary? Could colors other than red be used?
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Why must young cultures be used when doing a Gram stain?
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Explain how you would expect bacterial cells to look in the gram staining procedure if the following were made: a. Counterstain not used b. Iodine step left out c. Decolorizer left on too long or decolorizer not left on long enough d. Slide not heat-fixed before staining e. Cells from an old culture of Gram-positive bacteria are used
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