00:01
Hi, to answer this question, i wanted to draw a very simple diagram.
00:05
I couldn't think of a way to explain it other than just explaining it with these little tiny graphics.
00:12
So basically the reason why these two assays can have different numbers and different disagreeing results is because they are actually measuring different things in the protein.
00:24
So in the green, i just have written a hydroxyl group.
00:29
And so the idea here would be this carpicilic acid group on the end of any protein, or even in some of the side chains of a protein in myoflomen, that could bind to bradford reagent and would give you a certain concentration based on the binding to the bradford reagent and the cause and the color change.
00:53
And so that makes sense that that would happen for any protein, to be concentration dependent.
01:00
The soarate region is, or the sororate peak, is based on this porphorin ring that i drew here with the red blob that's myoglobin and this blue ring.
01:10
So the porphorin ring is this heem group that can bind to metals such as iron, right? and that's, that's this idea that with this ring, it's going to have an absorption spectrum and an emission spectrum.
01:27
And so that's what you're measuring with the sorate peak.
01:29
So there are two different things...