Worksheet #1: p-Nitrophenol Assay Wells | Volume of 0.12 ?mole/ml PNP (?ls) | Volume of Unknown #246 (?ls) | Volume of pH 4 Buffer B (?ls) | Volume of 0.1M, pH 11, Na carbonate (?ls) | Absorbance (at 405 nm) | Amount of PNP in tube (?moles) ---|---|---|---|---|---|--- A1 (Blank) | 0.0 | XXXX | 150?l | 150?l | 0.057 | 0.00 B1 (Blank) | 0.0 | XXXX | 150?l | 150?l | 0.067 | 0.00 A2 | 25?l | XXXX | 125?l | 150?l | 0.243 | 0.003 B2 | 25?l | XXXX | 125?l | 150?l | 0.237 | 0.003 A3 | 50?l | XXXX | 100?l | 150?l | 0.481 | 0.006 B3 | 50?l | XXXX | 100?l | 150?l | 0.380 | 0.006 A4 | 75?l | XXXX | 75?l | 150?l | 0.589 | 0.009 B4 | 75?l | XXXX | 75?l | 150?l | 0.552 | 0.009 A5 | 100?l | XXXX | 50?l | 150?l | 0.739 | 0.012 B5 | 100?l | XXXX | 50?l | 150?l | 0.819 | 0.012 A6 | 125?l | XXXX | 25?l | 150?l | 0.884 | 0.015 B6 | 125?l | XXXX | 25?l | 150?l | 0.866 | 0.015 A7 | XXXX | 25?l | 125?l | 150?l | 0.132 | B7 | XXXX | 25?l | 125?l | 150?l | 0.116 | A8 | XXXX | 50?l | 100?l | 150?l | 0.182 | B8 | XXXX | 50?l | 100?l | 150?l | 0.171 | A9 | XXXX | 75?l | 75?l | 150?l | 0.233 | B9 | XXXX | 75?l | 75?l | 150?l | 0.233 | A10 | XXXX | 100?l | 50?l | 150?l | 0.287 | B10 | XXXX | 100?l | 50?l | 150?l | 0.307 |
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