Would you expect the body to make the influenza HA protein faster with an injection of mRNA or DNA? A) mRNA because proteins are made directly from mRNA B) mRNA because it attaches to the amino acids C) DNA because it is the sole carrier of the instructions for proteins D) DNA because it binds with proteins to assemble them as the last step in protein synthesis A B C D
Added by James M.
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- mRNA (messenger RNA) carries the genetic code from DNA to the ribosomes, where proteins are synthesized. - DNA (deoxyribonucleic acid) contains the genetic information of an organism, but it does not directly participate in protein synthesis. Show more…
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Researchers have found a human protein (Protein X) that is a useful therapeutic for certain diseases. Protein X doesn't have any significant post-translational modifications and is a relatively small and water-soluble, globular protein. Therefore, Protein X can be expressed in its active form in the bacteria Escherichia coli. Expressing Protein X in and purifying it from E. coli will make it a much more affordable therapeutic. In order to do this, the researchers sequenced the mature mRNA that translates for Protein X in order to get the nucleotide sequence for the mature mRNA that codes for Protein X. They are now going to insert this sequence into a plasmid and transform that plasmid into E. coli, and then express and purify the protein. Which of the following should they also do before they insert the sequence into a plasmid and then into E. coli? Choose one or more: A. If the mRNA contains a UGA stop codon, then change it to a UAA stop codon. B. Add a sequence to encode an affinity tag (such as 6 histidine amino acids) at one end of the gene for Protein X. C. Change most of the AAA codons that encode for the amino acid lysine to AAG codons. D. Synthesize the DNA that is complementary to the mature mRNA sequence, and insert that into the plasmid instead of RNA.
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