Write a short paragraph about gel electrophoresis, which...

Write a short paragraph about gel electrophoresis, which should answer these questions below. 1. Why is DNA positioned at the black-cathode end? 2. What would happen if you misplace the DNA-containing wells at the anode end? 3. What is the charge on DNA? 4. How DNA fragments migrate on the gel?

Transcript

00:01 Gel electrophoresis is widely used laboratory technique for separating dna molecule based for separating dna molecule based on their size based on their size based on their size in this process dna is loaded in this process dna in this process dna is loaded loaded into well into well else at the anode end at the anode at the anode end which is positive terminal which is positive terminal of a gel matrix of gel of a gel matrix typically made up of a gross polycarbamide which is typically made up of a gross a gross polycarbamide carlomide but this the reason dna is position at the end -out end is because the reason the dna is position at the end -out end because because dna is negatively charged because dna is negatively charged dna is negatively charged due to its phosphate group due to its phosphate due to its phosphate group when electric current is applied when electric current is applied when electric current is applied the negatively charged dna fragment migrate negatively charged dna fragments will migrate negative negatively positively charged dna fragments will migrate towards positive terminal which is cathode which is basically represent by black electrode black electrode placing in the dna black electrode placing placing the dna at the anode and placing dna at anode and anode and placing dna at the anode and would typically result dna fragment to move in wrong direction wrong direction wrong direction away from gel away from away from gel and hence no outичe separation would occur and hence no separation no separation would occur no separation and hence no separation would occur.

05:08 As dna fragments moving faster dna fragments moving dna fragments moving faster creating creating a distinct band creating distinct creating a distinct band or pattern or the pattern that can be analyzed to determine that can be analyzed to determine to determine fragments and sized concentrations the segment size and concentration and concentration rations...

Question

Write a short paragraph about gel electrophoresis, which should answer these questions below. 1. Why is DNA positioned at the black-cathode end? 2. What would happen if you misplace the DNA-containing wells at the anode end? 3. What is the charge on DNA? 4. How DNA fragments migrate on the gel?

Please give Ace some feedback