1. Write the principle of HPLC.
2. Write the name of the major parts of HPLC instrument.
3. What is the difference between isocratic and gradient elution in HPLC?
4. What is the difference between normal and reversed phase HPLC?
5. What are the axes of chromatogram stands for?
6. Draw a standard calibration curve from data in table below using excel, then calculate the caffeine unknown concentration of peak area 522.
Peak area Caffeine Concentration (mg/L)
166 1
322 2
470 3
654 4
830 5