Question

You are a technician in a biochemistry lab running receptor binding experiments. The target membrane-bound receptor has been partially purified from mouse, rat, and human cell lines. Using the same radioactive ligand in a saturation binding assay for each species' receptor, you generate the binding data in the table. The dependent variable, Y, is the fraction of binding sites occupied by the ligand. Ligand concentration (nM) | Y for mouse receptor | Y for rat receptor | Y for human receptor 0.20 | 0.048 | 0.29 | 0.17 0.50 | 0.11 | 0.50 | 0.33 1.0 | 0.20 | 0.67 | 0.50 4.0 | 0.50 | 0.89 | 0.80 10 | 0.71 | 0.95 | 0.91 20 | 0.83 | 0.97 | 0.95 50 | 0.93 | 0.99 | 0.98 Determine the Kd for the human receptor in this binding experiment. human receptor Kd = nM Which receptor binds most tightly to this ligand?

          You are a technician in a biochemistry lab running receptor binding experiments. The target membrane-bound receptor has been partially purified from mouse, rat, and human cell lines. Using the same radioactive ligand in a saturation binding assay for each species' receptor, you generate the binding data in the table. The dependent variable, Y, is the fraction of binding sites occupied by the ligand.

Ligand concentration (nM) | Y for mouse receptor | Y for rat receptor | Y for human receptor
0.20 | 0.048 | 0.29 | 0.17
0.50 | 0.11 | 0.50 | 0.33
1.0 | 0.20 | 0.67 | 0.50
4.0 | 0.50 | 0.89 | 0.80
10 | 0.71 | 0.95 | 0.91
20 | 0.83 | 0.97 | 0.95
50 | 0.93 | 0.99 | 0.98

Determine the Kd for the human receptor in this binding experiment.

human receptor Kd = nM

Which receptor binds most tightly to this ligand?

$You are a technician in a biochemistry lab running receptor binding experiments. The target membrane-bound receptor has been partially purified from mouse, rat, and human cell lines. Using the same radioactive ligand in a saturation binding assay for each species' receptor, you generate the binding data in the table. The dependent variable, Y, is the fraction of binding sites occupied by the ligand. Ligand concentration (nM) | Y for mouse receptor | Y for rat receptor | Y for human receptor 0.20 | 0.048 | 0.29 | 0.17 0.50 | 0.11 | 0.50 | 0.33 1.0 | 0.20 | 0.67 | 0.50 4.0 | 0.50 | 0.89 | 0.80 10 | 0.71 | 0.95 | 0.91 20 | 0.83 | 0.97 | 0.95 50 | 0.93 | 0.99 | 0.98 Determine the Kd for the human receptor in this binding experiment. human receptor Kd = nM Which receptor binds most tightly to this ligand?$

Added by Cheryl C.

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