You carry out a PCR experiment, using genomic DNA as a template, and run the reaction out on an agarose gel. To your surprise, there are many (about a dozen) bands of different sizes present on the gel. Which of the following is the most likely explanation? There are restriction sites present in the genome that are interfering with your PCR experiment. The template DNA was heterozygous at the locus of interest You forgot to add the specific probe to the PCR reaction. You used primers that are complementary to sequences present at multiple different sites in the genome
Added by Brenda O.
Close
Step 1
Restriction sites in the genome would not interfere with PCR, as PCR does not rely on restriction enzymes. Show more…
Show all steps
Your feedback will help us improve your experience
Jenny Wu and 80 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
a) You have to run an important pcr reaction. Unfortunately you can't find any TAQ in the whole department. The only DNA poly DNA polymerase that is available is the Klenow fragment (E. coli DNA polymerase without exonuclease activity). Can you still run your pcr reaction using this DNA polymerase? Give a detailed explanation! b) You made a 1% agarose gel for the DNA fingerprinting experiment whereas a 2% agarose gel for this experiment. What could be the reason for it? Give a detailed explanation.
Jenny W.
The PCR reaction you performed was a success, and your target region (sequence highlighted in grey in section B above) of 160 bp in length has been amplified. You then subject your PCR amplicon to a restriction digest using the enzyme AluI, before cloning it into the AluI site of the 4.2 kb plasmid diagrammed below. BamHI at 300 EcoRI at 100 from 300 AluI at 200 from 100 EcoRI at 340 from 200 You now run the three RE digests of the recombinant plasmid only from question 2 on an agarose gel. Indicate your expected profile on the gel below. Clearly indicate the size of each fragment directly above it. EcoRI BamHI EcoRI + BamHI AluI _ +
Adi S.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
Watch the video solution with this free unlock.
EMAIL
PASSWORD