Question

You do serial dilutions of a bacteriophage stock. First, you move 1mL of stock into 9mL of sterile water and mix. Next, you put 1mL of that dilution into another 9mL of water to prepare your final phage dilution. You mix 100µL of your final phage dilution with 1mL of E. coli culture and 3 mL semi-soft agar then pour an agar overlay. The next day, you count 150 clear spots on the plate. Give the final dilution, relative to the stock, as a fraction: Give the concentration of the phage stock in scientific notation: x10^

          You do serial dilutions of a bacteriophage stock. First, you move 1mL of stock into 9mL of sterile water and mix. Next, you put 1mL of that dilution into another 9mL of water to prepare your final phage dilution.
You mix 100µL of your final phage dilution with 1mL of E. coli culture and 3 mL semi-soft agar then pour an agar overlay. The next day, you count 150 clear spots on the plate.

Give the final dilution, relative to the stock, as a fraction:

Give the concentration of the phage stock in scientific notation: x10^

Show more…

$You do serial dilutions of a bacteriophage stock. First, you move 1mL of stock into 9mL of sterile water and mix. Next, you put 1mL of that dilution into another 9mL of water to prepare your final phage dilution. You mix 100µL of your final phage dilution with 1mL of E. coli culture and 3 mL semi-soft agar then pour an agar overlay. The next day, you count 150 clear spots on the plate. Give the final dilution, relative to the stock, as a fraction: Give the concentration of the phage stock in scientific notation: x10^$

Added by Juana S.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

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Solved by Expert Jenny Wu

08/20/2023

Step 1

First, you move 1 mL of stock into 9 mL of sterile water. This is a 1:10 dilution, so the dilution factor is $\frac{1}{10}$. Show more…

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You do serial dilutions of a bacteriophage stock. First, you move 1 mL of stock into 9 mL of sterile water. Next, you put 1 mL of that dilution into another 9 mL of water. This prepares your final phage dilution. You mix 100 µL of your final phage dilution with 1 mL coli culture and 40 mL semi-soft agar, then pour an agar overlay. The next day, you count 50 clear spots on the plate. Give the final dilution, relative to the stock, as a fraction: Give the concentration of the phage stock in scientific notation:

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Transcript

00:02 You do sterile dilution of a bacteria phage stock.

00:05 First you move one ml of stock into nine ml of sterile water and mix.

00:10 Then you put one ml of that dilution into another nine ml of water.

00:17 And then this final phage dilution, you take one microliter of this final dilution with one ml of e .coli culture and three ml semi -soft agar.

00:32 Then pour the agar over it.

00:35 The next day you count 150 clear spots on the plate.

00:39 Give the final dilution relative to the stock as a fraction.

00:44 So the first dilution you take one ml and you add into nine ml of the water.

00:53 So you start out with one ml and you end up with one plus nine, ten ml.

00:58 So the volume increases ten times and the concentration drops ten times.

01:05 So this is a one to ten dilution.

01:06 So you repeat this process again.

01:10 You take one ml from the previous tube and add into another nine ml.

01:15 So one ml into ten ml.

01:21 Again this is a one to ten.

01:24 So these two are individual dilution.

01:26 The total dilution is going to be all the individual dilution multiplied together.

01:34 So one tenth times one tenth which is one to one hundredth...

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