5. You do serial dilutions on a water sample, and plate the dilutions on TSA plates. You count the colonies on each of the plates as follows: (Note: TMTC = too many to count) Dilution Number of CFUs 10^-2 TMTC 10^-3 TMTC 10^-4 352 10^-5 45 10^-6 6 10^-6 0 Based on these results, what is your estimate for the total number of CFUs/ml in the original sample?
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8. You have a urine sample from a patient that you suspect has a urinary tract infection. You make ten-fold dilutions of this sample as shown below, and then plate 0.5 ml (500 ̇̇µl) of the last dilution on a TSA plate. There are 45 colonies on the plate. How many CFUs/ml were in the original urine sample? 9. You do serial dilutions on a water sample and plate the dilutions on TSA plates. You count the colonies on each of the plates as follows: (Note: TMTC = too many to count) Dilution Number of CFUs 10^-2 TMTC 10^-3 TMTC 10^-4 352 10^-5 45 10^-6 6 10^-7 0 Based on these results, what is your estimate for the total number of CFUs/ml in the original sample?
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