You have 5ml of an overnight culture 2x108 cells perml how...

You have 5 ml of an overnight culture (2x10^8 cells per ml). How would you prepare a series of 1 or 10 ml dilutions (maximum dilution 1:100) to obtain a final 200 ml culture at 1x10^3 cells/ml? Provide work for each step.

Transcript

00:02 You have an overnight culture start with a concentration of two times 10 to 8 power cells per mill.

00:09 And you want to do a serious dilutions with a maximum dilution of 1 to 100 to obtain a final culture with 200 million liter and total volume with a concentration of 1 times 10 to the 3rd power cells per mil.

00:22 So you start with the stock again, 2 times 10 to 8 cells per mill.

00:27 The final concentration is 1 times 10 to the third power cells per mil.

00:33 First of all, we have to calculate our dilution factor.

00:36 The dilution factor is calculated by the stock concentration divided by the final concentration.

00:43 So two times 10 to the 8 is divided by 1 times 10 to the 3rd equals 2 times 10 to the 5th power.

00:51 So to do a dilution from stock to the final concentration, you must do a one to 200 ,000 dilution.

01:03 So now let's see how can we do this 1 to 200 ,000? because you can't do it in one step.

01:11 It will not be accurate.

01:13 So we actually did it in four steps.

01:15 The very first step, you will take one millimeter out from the stock and add that into a small tube that contained 0 .9 millimeter of medium.

01:24 Now, the volume goes from 0 .1 to 1.

01:28 The volume increased 10 times, the concentration drops 10 times.

01:32 So we label it as a, tube a, and this is a 1 to 10 dilution according to the stock solution.

01:42 Now, the second step, i take another tube and again filled up with 0 .9 militer of medium.

01:48 Now this time i take 0 .1 millimeter out from tube a.

01:52 So again, the concentration will drop 10 times because the volume goes from 0 .1 to 1 again, the volume increased 10 times.

02:01 So now this new tube, we called it tube b...

Question

You have 5 ml of an overnight culture (2x10^8 cells per ml). How would you prepare a series of 1 or 10 ml dilutions (maximum dilution 1:100) to obtain a final 200 ml culture at 1x10^3 cells/ml? Provide work for each step.

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