You have been given a soil sample which has a predominant population of Bacillus subtilis. There are other bacteria as well in the soil sample. You have been asked to isolate Bacillus subtilis from the mixed population using pour plate technique. Describe how would you achieve this?
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Step 2: Pour the mixture into an empty plate and allow it to solidify to form a pure culture. Step 3: Isolate Bacillus subtilis by using special media with specific chemical or physical agents for enrichment or selection. Step 4: Serially dilute the culture and Show more…
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8. You have been given a soil sample which has a predominant population of Bacillus subtilis. There are other bacteria as well in the soil sample. You have been asked to isolate Bacillus subtilis from the mixed population using pour plate technique. Describe how would you achieve this? 9. What is a potential use for creating lawns of bacteria? Describe with the help of an experiment.
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You want to know the relative abundance of Bacillus species in a soil sample. You divide your soil sample into two equal samples A and B. You heat sample A at 80C for 15 minutes, but you do not heat sample B. You plate both your heated soil sample A and your unheated soil sample B onto Glucose Nutrient Agar (GNA). After incubation, you count 35 colonies on your GNA-sample A plate and you count 70 colonies on your GNA-sample B plate. What percentage of colonies on the GNA-sample B plate are likely to be the product of Bacillus spores?
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The bacterium $P$ seudomonas aeruginosa is cultured with an initial population of approximately 10,000 active organisms. The population of active bacteria increases up to a point. Then, due to a limited food supply and an increase of waste products, the population decreases. The population $P$ can be approximated by $$P=-1718 t^{2}+82,000 t+10,000$$ where $t$ is the number of hours since the culture was started. a. Use the model to approximate the population after $22 \mathrm{hr}$. Round to the nearest thousand. b. Use the model to approximate the population after 1.5 days. Round to the nearest thousand. c. Use the model to approximate the population after $48 \mathrm{hr}$. Round to the nearest thousand.
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