You have chosen to cut your DNA and plasmid with the HindIII restriction enzyme. This enzyme recognizes the sequence AAGCTT and makes a cut between the two A bases. This will generate DNA fragments with an overhang sequence of AGCT. Write the sequence of the single-stranded overhang of your plasmid DNA that would hydrogen bond with this sticky end you generated on your cut DNA.
Added by Kurt H.
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Step 1: The HindIII restriction enzyme cuts the DNA at the sequence AAGCTT, generating sticky ends with the sequence AGCT. Show more…
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You have a piece of DNA with the sequence shown below: 5'-AAAGTCGCTGGAATTCACTGCATCCCCGGGGCTATATATGAATTCGATGCGTACTTGGCACG-3' 3'-TTTCAGCGACCTTAAGTGACGTAGGGGCCCCGATATATACTTAAGCTACGCATGAACCGTGC-5'. You cut this fragment with the restriction enzyme EcoRI. The recognition site for EcoRI is shown below: GAATTC 3' CTTAAG 5' EcoRI cuts at the site and in the manner indicated by the scissors to yield fragments with overhanging ends AATTC 3' 3'-CTTAA G 5'. Below, show the fragments that would result from the restriction digest of that piece of DNA by EcoRI. Show all the base pairs and the overhanging ends of the fragments:
Farhan A.
The restriction enzyme BamHI recognizes the sequence GGATCC and cuts the DNA between the two G's. Given the following segment of double-stranded DNA, draw the sticky ends that result from cleavage with this enzyme.
Sri K.
When a typical restriction enzyme cuts a DNA molecule, the cuts are uneven, giving the DNA fragments single-stranded ends. These ends are useful in recombinant DNA work because a. they enable a cell to recognize fragments produced by the enzyme. b. they serve as starting points for DNA replication. c. the fragments will bond to other fragments with complementary ends. d. they enable researchers to use the fragments as molecular probes.
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