You have human cells that you perform flourescent in situ hybridization on using a yellow probe that detects XIST DNA and a red probe that specifically detects XIST RNA. How many nuclear spots of hybridization with the XIST probes for DNA and RNA will be observed in the following situations? Tissue culture cells derived from an XY male: XIST DNA A XISTRNA A
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Step 1: In an XY male, one X chromosome is active and the other is inactive. Show more…
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You have human cells that you perform fluorescent in situ hybridization on using a yellow probe that detects XIST DNA and another red probe that specifically detects XIST RNA. How many nuclear spots of hybridization with the XIST probes for DNA and RNA will be observed in the following situations: Male tissue culture cells. XIST DNA {A} XIST RNA {B} Female tissue culture cells (2X) that have an autosome with the X chromosome inactivation centre. XIST DNA {C} XIST RNA {D}. A?, B?, C?, D? Choices for all of them are: 0 1, 2, 3, 4, 5
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Answer the following questions regarding X-inactivation: What are XIST and TISX? (1 pt) 46,XX 46,XY 47,XXY 49,XXXXX 49,XXXXY The image on the right is a gel blot of RNA from cells with different chromosomal make-ups. The spots indicate the amount of XIST in each of the cells. Explain why there is more XIST produced in some cells based on their chromosomal composition: (1 pt) A DNA fragment with the following base sequence has some cytosine bases that are methylated (indicated by *), and others that are unmethylated. To determine the location of methylated and unmethylated cytosines, researchers sequenced this fragment both with and without treatment with sodium bisulfite. Give the sequence of bases that will be read with and without bisulfite treatment (1 pt) ATCGC*GTTAC*ATTGC*ATCA
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How many Barr bodies (condensed X chromosomes) would you predict in this boy's cells? One per somatic cell Three per somatic cell Two per somatic cell Either two or three depending on the tissue type None
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