You have isolated genomic DNA in a 50 μl final volume. You add 5 μl of the DNA solution to a 1 ml final volume and measure the OD260 to be 0.04. How much DNA is in the cuvette and how much do you have in total?
Added by Jon B.
Step 1
Given that the DNA concentration is 50 micrograms per milliliter and the dilution factor is 200, we can calculate the DNA concentration in the cuvette as follows: DNA concentration in cuvette = 50 micrograms/mL x 200 = 10000 micrograms/mL Show more…
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