You load two samples of linear DNA on an agarose gel. After running the gel, you observe that the two samples traveled the same distance from the wells. From this you can conclude? The two samples contained DNA of similar size (length in bp) The two samples contained similar amounts of DNA (weight in mg) The two samples contained DNA of similar concentration (in mM) The two samples contained similar volumes of DNA (in µL)
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Step 1: The observation that the two samples traveled the same distance on the agarose gel indicates that the DNA in both samples is of similar size (length in base pairs). Show more…
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ii) Based on the gel image above, from Sample 1 to Sample 5, DNA fragment size in each sample is increasing / decreasing /relatively similar (circle the correct answer). iii) Assuming you measure the DNA concentration of these samples using a NanoDrop instrument. All five samples have similar DNA concentration (e.g. ~400ng/Δl). Which sample has the highest DNA integrity? If you think they all have the same DNA integrity, write "all the same" in the space below. Sample ______ or __________ iv) The protocol and reagents used in all five extractions were the same. What is the best explanation for the different electrophoresis banding patterns observed in this gel image?
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You extract DNA from a sample and estimate it to be at a concentration of 20 mg/mL. You would like to load 20 µL of a 50 ng/µL in a well in a 1% agarose gel. Write out a procedure that will allow you to do this.
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Like a ladder. Circular DNA from SV40 virus was isolated and subjected to gel electrophoresis. The results are shown in lane $\mathrm{A}$ (the control) of the adjoining gel patterns. (a) Why does the DNA separate in agarose gel electrophoresis? How does the DNA in each band differ? The DNA was then incubated with topoisomerase I for 5 minutes and again analyzed by gel electrophoresis with the results shown in lane B. (b) What types of DNA do the various bands represent? Another sample of DNA was incubated with topoisomerase I for 30 minutes and again analyzed as shown in lane $\mathrm{C}$. (c) What is the significance of the fact that more of the DNA is in slower-moving forms? FIGURE CANT COPY
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