you obtain a value of 345000 cells/mL and want to seed 4,000 cells/cm^2 in a new T-25 flask. How much cell solution should you add to your new flask?
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Sri K.
Assume that using the trypan blue exclusion assay, you counted the total number of cells in your culture as 4.2 x 10^4 cells/ml. You diluted your culture 1:42 and then seeded 100 μl per well into a 96-well plate. What is the final cell density per well?
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