You obtained the following raw data when setting up a Bradford standard curve:
BSA (mg/ml) Absorbancy 595nm
0 0.225
1 0.310
2 0.420
3 0.510
4 0.610
5 0.720
6 0.810
7 0.915
8 0.950
9 0.980
10 0.990
After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.594 was obtained.
Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).