You perform a viral plaque assay. You pipette 250ul of unknown viral samples in a dilution series in the plate and obtain the following result. What is the viral titre in PFU/mL? Group of answer choices None of the above 7.2 x 10^4 PFU/mL 8 x 10^5 PFU/mL 7.2 x 10^5 PFU/mL 8 x 10^4 PFU/mL 8 x 10^6 PFU/mL
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Jenny W.
After performing a plaque assay, where you plated 200 µl of each dilution, you count 206 plaques at a dilution of 10^-7. Determine the number of plaque forming units (pfu) per ml of sample. pfu/ml = [# plaques/plating factor] x dilution factor. If you have 50,000 µl of your sample, what is the total amount of pfu you have available? Note: How much volume you would use in your infection, how you calculated this volume, and how you determined the amount of virus you need to perform the assay. We cannot measure volumes <1 µl.
Tracy L.
0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.9 mL diluent virus filtrate P200 075 apply to each test surface Three days after the plaque assay was infected and aliquots of virus applied to surfaces, Velma viewed the results of the 6-well plate: there were 32 plaques in well F and too many plaques to count in wells A-E. Based on the volume shown for the P200 and the PFU/mL value determined by the plaque assay, the titer of virus that each surface would have received was 3.20 x 10^ 5 PFU. Use good scientific notation: type your answer in the first box in the format n.nn and then type the exponent into the second box.
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