You ran the gel electrophoresis of your PCR products without any controls. Identify and describe the outcome of potential positive and negative controls that you could run to verify that the bands you are seeing are due to the amplification of the extracted DNA from the PCR reactions performed. This is for a genetics lab on DNA Extraction, Amplification, Digestion and Analysis via Gel Electrophoresis.
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The gel below shows a result of the experiment you designed using the DNA of three different individuals (S1-S3). It also shows a negative (C-) and a positive (C+) control, as well as a DNA marker. It is indicated where the restriction enzyme Fnu4H1 was used to cut your PCR products. DNA marker C- C+ S1 S2 S3 Base pair 750 400 300 200 100 50 Enzyme - + + + + Lane 1 2 3 4 5 DNA marker = DNA of known sizes. C- = negative control: undigested PCR product deriving from TAS2R38 gene. C+ = positive control: digested PCR product of known sequence from a strong taster with a single Fnu4H1 restriction site. S1 - S3 = results of different individuals.
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The image below is of a PCR reaction from genomic DNA, from several different samples. C+ is the positive control and C- is the negative control. 1. What is the most likely size of the PCR product? 2. What would be an appropriate positive control for the PCR in this experiment? 3. What would be an appropriate negative control for the PCR in this experiment? 4. In sample 4, two bands are clearly visible. What is a likely explanation for the two bands? 5. Which sample would you send for sequencing?
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You ran an experiment in the lab that produced the following gel-electrophoresis results. Which well(s) were negative control and did not contain any DNA fragments? Select all that apply:
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