You set up two tubes of E. coli with an inoculum and a defined medium. In both tubes, you add 1% glucose as a carbon and energy source with minimal media. You shake one tube to aerate the medium and leave one still to force E. coli into an anaerobic environment. This strain only has an aerobic electron transport chain. Predict the relative growth of the two cultures and how much glucose will be left in each after an overnight 37°C incubation.
Added by Justin W.
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In the aerobic environment (shaken tube), E. coli can use its aerobic electron transport chain to generate ATP through oxidative phosphorylation. This allows for efficient energy production and faster growth. Show more…
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