Question

You study ligand binding to two proteins, A and B (aka macromolecules, M). You measured the concentration, [Mx], of the ligand-bound form of the protein at various ligand concentrations, [x]. The data are summarized in the two tables below. Note that you do not know the total concentration of the protein, but you see that adding more ligand noticeably change the bound-protein concentration as show below. Protein A [x], ?M | [Mx], ?M 0 | 0.000 5 | 0.040 10 | 0.067 50 | 0.133 200 | 0.282 500 | 0.554 2000 | 1.899 5000 | 4.609 Protein B [x], ?M | [Mx], ?M 0 | 0.00 15 | 0.21 30 | 0.33 50 | 0.41 100 | 0.67 300 | 0.77 1000 | 0.79 3000 | 0.81 a) Determine the Kd values for each of the proteins, if you can. Explain your answers (why you can or why you cannot). b) Which of the two proteins binds the ligand tighter? Explain.

          You study ligand binding to two proteins, A and B (aka macromolecules, M). You measured the concentration, [Mx], of the ligand-bound form of the protein at various ligand concentrations, [x]. The data are summarized in the two tables below. Note that you do not know the total concentration of the protein, but you see that adding more ligand noticeably change the bound-protein concentration as show below.

Protein A
[x], ?M | [Mx], ?M
0 | 0.000
5 | 0.040
10 | 0.067
50 | 0.133
200 | 0.282
500 | 0.554
2000 | 1.899
5000 | 4.609

Protein B
[x], ?M | [Mx], ?M
0 | 0.00
15 | 0.21
30 | 0.33
50 | 0.41
100 | 0.67
300 | 0.77
1000 | 0.79
3000 | 0.81

a) Determine the Kd values for each of the proteins, if you can. Explain your answers (why you can or why you cannot).
b) Which of the two proteins binds the ligand tighter? Explain.

You study ligand binding to two proteins, A and B (aka macromolecules, M). You measured the concentration, [Mx], of the ligand-bound form of the protein at various ligand concentrations, [x]. The data are summarized in the two tables below. Note that you do not know the total concentration of the protein, but you see that adding more ligand noticeably change the bound-protein concentration as show below.

Protein A
[x], ?M | [Mx], ?M
0 | 0.000
5 | 0.040
10 | 0.067
50 | 0.133
200 | 0.282
500 | 0.554
2000 | 1.899
5000 | 4.609

Protein B
[x], ?M | [Mx], ?M
0 | 0.00
15 | 0.21
30 | 0.33
50 | 0.41
100 | 0.67
300 | 0.77
1000 | 0.79
3000 | 0.81

a) Determine the Kd values for each of the proteins, if you can. Explain your answers (why you can or why you cannot).
b) Which of the two proteins binds the ligand tighter? Explain.

Added by Ralph J.

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