You used the Bradford (Coomassie) assay to determine the concentration of a histone protein. You used bovine albumin as your standard. You determined the histone protein concentration to be 1.5 mg/ml. When you used the same histone protein as your standard, you determined the true histone protein concentration to be 1 mg/ml. Explain the discrepancy. Chegg.
Added by Manuel F.
Step 1
Bovine albumin and histone proteins have different amino acid compositions and structures, which can affect how the Coomassie dye binds to them and consequently the color change observed in the Bradford assay. Show more…
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You use the Bradford (Coomassie) assay to determine the concentration of a histone protein. You use bovine albumin as your standard. You determine the histone protein concentration to be 3 mg/ml. When you use the same histone protein as your standard, you determine the histone protein concentration to be 5 mg/ml. Explain the discrepancy (pick ONE answer): a. The histone protein has fewer basic amino acids than albumin. b. The histone protein has more acidic amino acids than albumin. c. The histone protein has more basic amino acids than albumin. d. The histone protein contains more methionine than albumin.
Sri K.
You would like to determine the concentration of purified protein using a Bradford Assay; you generate the following standard curve. Calculate the concentration of your protein (show your calculations and report your answer to three significant digits). Note: The Abs of your protein that was diluted by a factor of 10 is 1.25.
David C.
To measure the amount of protein in your unknown samples, you first need to calibrate the change in Bradford reagent absorbance induced by different amounts of protein. You already did this in the lab when you determined the extinction coefficient of BSA bound to the Bradford Reagent (remember?). Question: In your own words, briefly explain the theory behind how we will determine the total protein concentration in our samples. Procedure: Determining the protein concentrations of your samples. The goal of this part is to determine the protein concentration of your samples by first diluting them.
Jenny W.
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