You want to insert a gene into a plasmid in order to...

You want to insert a gene into a plasmid in order to transform E. coli and give it new capabilities. You have both the gene and the plasmid available. In order to put the gene into the plasmid, you would first need to treat both with the same ______. RNA polymerase DNA polymerase restriction enzyme ligase

Transcript

00:01 Hello again and welcome to this video where we're going to learn about gene insertion.

00:08 Gene insertion, if you want to transform an organism, you have to insert the gene into that organism.

00:16 And for you to insert the gene into that organism, you have to really use a vehicle, molecular vehicle.

00:24 A vehicle is a kind of molecule that will enable you inside the gene into a genoma of a molecule.

00:31 Organism of interest and most vehicles that are using molecular biology are known as plasmids.

00:38 Plasmids are circular, extrachromosomal genetic elements that can be used to make sure that the gene of interest is inserted where it needs to be.

00:53 Placids are circular, okay? plasmids are circular, which means they can be cut at an even point.

01:02 And for you to cut a plasmid and insert the gene correctly, you need to use terms, structures or molecules we call molecular scissors.

01:23 Okay.

01:24 And these are what you call restriction enzymes.

01:29 And examples of restriction enzymes are like you can use ikorai, from astitchin enzymes.

01:41 Chacholice strain 1 can use bacillus, malolucifurcians, strain h, can use amyfluous influenza influenza strain 3, okay? these are but restriction enzymes derived from bacteria, okay? so for you to cut this plasmid, remember we need to have one restriction enzyme for both, one restriction enzyme for both plasmid and the gene of interest...

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