Question

4. You want to PCR the target sequence in the genomic DNA shown below. What method is used in the lab to cause the two strands of genomic DNA to come apart (= denature=break all the H-bonds holding the strands together)? . Genomic DNA Target sequence 1 Denaturation 2 Annealing 3 Extension If the two strands were all A's and all T's as shown, what two primers might you have synthesized in order to do PCR? (circle them) 5'-AAAAAA-3' and 5'-GGGGGG-3' 5'-AAAAAA-3' and 5'-TTTTTT-3' 5'-CCCCCC-3' and 5'-GGGGGG-3' 5'-TTTTTT-3' and 5'-CCCCCC-3' Draw where those primers would bind on the two denatured DNA strands. 5. A figure of a "generic" gene is shown below with its direction of transcription. Draw a box around and label the promoter and the terminator of this gene. What enzyme synthesizes the RNA product? . Where in the cell would this occur? . The transcribed region DNA Initiation of transcription occurs at what part of a gene? . In addition to the main enzyme for transcription (which you filled in on the previous question), what type of proteins are required to initiate transcription in eukaryotic cells? . These additional proteins are important because they allow for very complex (replication, regulation, recreation, resignation) of gene expression. 6. RNA processing takes place in the eukaryotic nucleus and results in modification of the 5' and 3' ends of eukaryotic pre-mRNAs. What other sort of surprising processing event occurs on almost all pre-mRNAs? . In that processing event, the parts of a pre-mRNA that will be included in the final "mature" mRNA (called the ) are joined together and the parts of the pre-mRNA that are removed (called the ) are cut out and thrown away.

4. You want to PCR the target sequence in the genomic DNA shown below. What method is used in the lab to cause the two strands of genomic DNA to come apart (= denature=break all the H-bonds holding the strands together)? ______________.

Genomic DNA
Target sequence
1 Denaturation
2 Annealing
3 Extension

If the two strands were all A's and all T's as shown, what two primers might you have synthesized in order to do PCR? (circle them)
5'-AAAAAA-3' and 5'-GGGGGG-3'
5'-AAAAAA-3' and 5'-TTTTTT-3'
5'-CCCCCC-3' and 5'-GGGGGG-3'
5'-TTTTTT-3' and 5'-CCCCCC-3'

Draw where those primers would bind on the two denatured DNA strands.

5. A figure of a "generic" gene is shown below with its direction of transcription. Draw a box around and label the promoter and the terminator of this gene. What enzyme synthesizes the RNA product? ______________. Where in the cell would this occur? ______________.

The transcribed region
DNA

Initiation of transcription occurs at what part of a gene? ______________. In addition to the main enzyme for transcription (which you filled in on the previous question), what type of proteins are required to initiate transcription in eukaryotic cells? ______________. These additional proteins are important because they allow for very complex (replication, regulation, recreation, resignation) of gene expression.

6. RNA processing takes place in the eukaryotic nucleus and results in modification of the 5' and 3' ends of eukaryotic pre-mRNAs. What other sort of surprising processing event occurs on almost all pre-mRNAs? ______________. In that processing event, the parts of a pre-mRNA that will be included in the final "mature" mRNA (called the ______________) are joined together and the parts of the pre-mRNA that are removed (called the ______________) are cut out and thrown away.

4. You want to PCR the target sequence in the genomic DNA shown below. What method is used in the lab to cause the two strands of genomic DNA to come apart (= denature=break all the H-bonds holding the strands together)? .

Genomic DNA
Target sequence
1 Denaturation
2 Annealing
3 Extension

If the two strands were all A's and all T's as shown, what two primers might you have synthesized in order to do PCR? (circle them)
5'-AAAAAA-3' and 5'-GGGGGG-3'
5'-AAAAAA-3' and 5'-TTTTTT-3'
5'-CCCCCC-3' and 5'-GGGGGG-3'
5'-TTTTTT-3' and 5'-CCCCCC-3'

Draw where those primers would bind on the two denatured DNA strands.

5. A figure of a "generic" gene is shown below with its direction of transcription. Draw a box around and label the promoter and the terminator of this gene. What enzyme synthesizes the RNA product? . Where in the cell would this occur? .

The transcribed region
DNA

Initiation of transcription occurs at what part of a gene? . In addition to the main enzyme for transcription (which you filled in on the previous question), what type of proteins are required to initiate transcription in eukaryotic cells? . These additional proteins are important because they allow for very complex (replication, regulation, recreation, resignation) of gene expression.

6. RNA processing takes place in the eukaryotic nucleus and results in modification of the 5' and 3' ends of eukaryotic pre-mRNAs. What other sort of surprising processing event occurs on almost all pre-mRNAs? . In that processing event, the parts of a pre-mRNA that will be included in the final "mature" mRNA (called the ) are joined together and the parts of the pre-mRNA that are removed (called the ) are cut out and thrown away.

Added by James T.

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