Question

1. You will need 60 ml of the cell suspension at a concentration of 1 x 10^6 cells/ml to complete your MTT Assay. What volume of cells will you need from your cell suspension containing 7.2 x 10^8 cells/ml? (show your calculations) 2. Prepare 150 ml of a 50mM stock solution of Epirubicin (MW=543 g/mol) and Cyclophosphamide (251 g/mol). (show your calculations) 3. If you add 10 µl of your stock solution to 200 µl of your cells suspension, what is the final concentration of drug your cells are being exposed to? 4. The detergent reagent in the MTT Assay needs to be diluted from a 50X stock solution to a working dilution (1X) before using it. If you need 10 µl to add to each well of the 96 well plate, how much detergent reagent will you need to prepare, and how much of the stock solution will you need to use? 5. The Principal Investigator of your research project has determined that a larger sample volume is needed for your MTT Assay. If you increase the cell suspension volume from 200 µl to 250 µl, how much MTT reagent will you need to add to each well if you were adding 10 µl before?

          1. You will need 60 ml of the cell suspension at a concentration of 1 x 10^6 cells/ml to complete your MTT Assay. What volume of cells will you need from your cell suspension containing 7.2 x 10^8 cells/ml? (show your calculations)
2. Prepare 150 ml of a 50mM stock solution of Epirubicin (MW=543 g/mol) and Cyclophosphamide (251 g/mol). (show your calculations)
3. If you add 10 µl of your stock solution to 200 µl of your cells suspension, what is the final concentration of drug your cells are being exposed to?
4. The detergent reagent in the MTT Assay needs to be diluted from a 50X stock solution to a working dilution (1X) before using it. If you need 10 µl to add to each well of the 96 well plate, how much detergent reagent will you need to prepare, and how much of the stock solution will you need to use?
5. The Principal Investigator of your research project has determined that a larger sample volume is needed for your MTT Assay. If you increase the cell suspension volume from 200 µl to 250 µl, how much MTT reagent will you need to add to each well if you were adding 10 µl before?

1. You will need 60 ml of the cell suspension at a concentration of 1 x 10^6 cells/ml to complete your MTT Assay. What volume of cells will you need from your cell suspension containing 7.2 x 10^8 cells/ml? (show your calculations)
2. Prepare 150 ml of a 50mM stock solution of Epirubicin (MW=543 g/mol) and Cyclophosphamide (251 g/mol). (show your calculations)
3. If you add 10 µl of your stock solution to 200 µl of your cells suspension, what is the final concentration of drug your cells are being exposed to?
4. The detergent reagent in the MTT Assay needs to be diluted from a 50X stock solution to a working dilution (1X) before using it. If you need 10 µl to add to each well of the 96 well plate, how much detergent reagent will you need to prepare, and how much of the stock solution will you need to use?
5. The Principal Investigator of your research project has determined that a larger sample volume is needed for your MTT Assay. If you increase the cell suspension volume from 200 µl to 250 µl, how much MTT reagent will you need to add to each well if you were adding 10 µl before?

Added by Jessica J.

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