Question

You are counting cells that you have prepared from a mouse spleen. Using the hemocytometer, you determine that you have 5.8 x 10^6 cells/ml in your final suspension of cells. These cells are in a volume of 20 ml. Answer the following questions: How many total spleen cells did you obtain from this mouse (2 points)? You want to stain 10^6 cells with an antibody to CD4. How many milliliters of the mouse spleen cell suspension do you need to use (2 points)? The antibody to CD4 (anti-CD4) is available at a stock concentration of 500 ug/ml. When you stain the 10^6 cells from part b, you will do so in a final volume of 0.1 ml, using an antibody concentration of 10 ug/ml. How much of the stock antibody do you need to add to your 0.1 ml solution to give you the appropriate final concentration (2 points)?

          You are counting cells that you have prepared from a mouse spleen. Using the hemocytometer, you determine that you have 5.8 x 10^6 cells/ml in your final suspension of cells. These cells are in a volume of 20 ml. Answer the following questions:
How many total spleen cells did you obtain from this mouse (2 points)? 
You want to stain 10^6 cells with an antibody to CD4. How many milliliters of the mouse spleen cell suspension do you need to use (2 points)? 
The antibody to CD4 (anti-CD4) is available at a stock concentration of 500 ug/ml. When you stain the 10^6 cells from part b, you will do so in a final volume of 0.1 ml, using an antibody concentration of 10 ug/ml. How much of the stock antibody do you need to add to your 0.1 ml solution to give you the appropriate final concentration (2 points)?

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Added by Alvaro C.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

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Solved by Expert Farhan Anwar

01/12/2023

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Count the cells in a hemocytometer: 5.8 x 106 cells/ml Show more…

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You are counting cells that you have prepared from a mouse spleen. Using the hemocytometer, you determine that you have 5.8 x 10^6 cells/ml in your final suspension of cells. These cells are in a volume of 20 ml. Answer the following questions: How many total spleen cells did you obtain from this mouse (2 points)? You want to stain 10^6 cells with an antibody to CD4. How many milliliters of the mouse spleen cell suspension do you need to use (2 points)? The antibody to CD4 (anti-CD4) is available at a stock concentration of 500 ug/ml. When you stain the 10^6 cells from part b, you will do so in a final volume of 0.1 ml, using an antibody concentration of 10 ug/ml. How much of the stock antibody do you need to add to your 0.1 ml solution to give you the appropriate final concentration (2 points)?

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Transcript

00:01 Okay, so we're counting cells prepared from a mouse spleen.

00:03 So we determine that there is 5 .8 times 10 to the 6 cells per mil in the suspension, and the total suspension volume is 20 mils.

00:18 So basically you multiply this by 20 milliliters.

00:23 You cancel the mills, so when you multiply these together, when you multiply these together, so that gives us a total number of cells.

00:48 Of 1 .16 times 10 to...

00:52 1, 2, 3, 4, 6, and 8 times 10 to the 8th cells.

00:59 So this is our total number.

01:02 Okay? let's see.

01:06 You want to stain 106 cells with an antibody to cd4.

01:11 How many milliliters of the cell suspension do you need? so you want 106 cells.

01:21 So if you look at it, 106 is another way to write that is 1 .06 times 10 of the 2.

01:31 So if you take a look, what we essentially do now is divide 1 .16 times 10 to the 8th by 1 .06 times 10 to the 2.

01:48 You divide that, and we get, and i'm sorry, i miss, let's do it this way, i, i inverted the numerator and denominator.

02:09 Sorry, you divide 1 .06 times it out of the two by 1 .16 times 10 to the eighth.

02:17 And that gets you, let's see, 106 divided by one more.

02:31 It gets us essentially 9 .14 times 10 to the minus 7.

02:49 Okay.

02:51 So that's the ratio that we need.

02:54 So another way the right data is actually basically that.

03:03 Now we multiply this by 20.

03:14 We get 1 .8 times 10 to the minus 5th milliliters...

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